Table of Contents. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The following document is intended to demonstrate setup of this. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Multimode Readers Comparison Chart. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The plate reader. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. Refurbished. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Description 1. Step 4: Incubate at room temperature for 1 hour, protected from light. Table of Contents. 314 low lamp level warning. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Set the excitation at 531 nm and emission at 595 nm. 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. FlexStation® 3 Multi-Mode Microplate Reader. . For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Five preferred modes for diverse bioresearch applications. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Softmax Pro GxP Software 6. 2. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. . Exhibit with us {{>currencyLabel}} Back. For example, microplates containing different samples can be read using the312 gain calibration failed. spr SpectraMax iD3 SpectraTest ABS2 v1. The following document is intended to demonstrate setup of this. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). . UV/Vis Photometric Performance:. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). The SpectraMax M5 Multi-Mode. Softmax Pro GxP Software 6. I have been using the SpectraMax M5 since 2011. . 1321N1, a human astrocytoma cell line, expresses. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The following document is intended to demonstrate setup of this instrument. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Control Panel The control panel houses the instrument status LEDs and the microplate. , ~488 nm), has the optical. Step 2. ) Click the "OK" button on SoftMax Pro Setup dialog box . The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The assay applies fluorescence polarization to quantify Fc-containing IgG. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Spectral Range. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SpectraMax Injector Cartridge with SmartInject™ Technology. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. 3: The cuvette chamber. $4,499. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. The Spectr. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. . Recertification is recommended every two years. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. Wavelength Range. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The assay window spanned four orders of magnitude. . Description 1. 304 signal level saturation. Description 1. The M5 can read volumes down to 2uL with the uMax Low Volume. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. 7" color, capacitive touch screen. 1. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. D 1 1. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. . SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Softmax Pro GxP Software 6. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. Proceed with the following steps to optimize the instrument: 1. Description 1. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 72 MB. EUROPE. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. The guide also covers the software features, the specifications, and the accessories of the systems. 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 0 out of 5 $ 17,999 $ 16,995. The. . 1. The following document is intended to demonstrate setup of this instrument. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. . Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. We would like to show you a description here but the site won’t allow us. No accessories are needed and SoftMax® Pro. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. . Instructions on the proper use of the SpectraMax M5 Plate Reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. com A. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Table of Contents. SpectraMax Microplate Readers. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The chamber. SOFTmax PRO UserÕs Manual iii Contents Figures . 4. 00 USD. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 1. 312 gain calibration failed. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6. When the correct COM port is selected, the. . 4. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. KeySpan adapter for SoftMax Pro and SpectraMax readers. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. UV/Vis Photometric Performance:. Step 1: Open the SoftMax Pro IMAP FP protocol. 313 reference gain check fail. . The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Statistical analyses The statistical analyses were performed using Graph Prism version 7. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. com A. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Maximum signal with lowest background. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. the IMAP Platform and smoothly transition into high-throughput screening. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Fluorescence Microplate Reader Comparison Chart. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Wide range of applications. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Wash Buffer Concentrate was diluted 10-fold. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. 3. 4. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. . The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. This application note describes how to. e. All reagents were brought to room temperature before use. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Cavicide (Warehouse, Cat # 79300360) 6. Table of Contents. Set the excitation at 531 nm and emission at 595 nm. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Optimal instrument settings are the same for all three readers. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). edu) to be added as a trained user in iLab. The dual monochromator optics allow. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Step 1: Open the SoftMax Pro IMAP FP protocol. There were 12 replicate wells per dilution in both 96-well and 384- well plates. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). View the Molecular Devices website. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 2. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. free shipping. 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. 2. Read More. ATP standard curves read on SpectraMax microplate readers. Also refer to this Multimode Reader Comparison Chart. 3. The SpectraMax M5 is a microplate reader that can be used for various applications,. 3. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Step 1. HTRF-compatible SpectraMax Multi-Mode Readers. 19. 00 USD. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. SpectraMax M2 and SpectraMax M2e are controlled by an external computer running SoftMax Pro software which provides integrated instrument control, data display, andQualify the fluorescence performance of the SpectraMax . 313 reference gain check fail. D 1 1. 2. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. SpectraMax M5, Analyst HT or Analyst GT. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. VWR offers biophotometers for your lab's needs. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 0 out of 5 $ 5,999. Three kits covering a wide range of dsDNA concentrations. . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 315 can't find zero order. When a feature or capability applies to only certain readers, this exception is noted. Sensitive fluorescent quantitation of DNA with the Quant-iT. Example: [Drawer]. 01) + 0. 5. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. . The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. We would like to show you a description here but the site won’t allow us. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 190-1000 nm, tunable in 1 nm increments. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Description 1. Add to Cart. e. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Select the fluorescence option and read the total fluorescence. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. When the user applies a set temperature of 37°C, the top is heated to 37. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 9000-0756. Monday to Friday 7am - 5pm Pacific Time. Examples: “Press carefully to engage,” “ Do not. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. In the M2, M5 and Plus. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Spectral Range. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. These robust readers have been placed in labs from. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 306 plate air cal. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Highly selective for dsDNA over ssDNA or RNA. Table of Contents. 7 nM EGF, the EC 80 concentration determined from. . High tolerance for contaminants. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 3. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. The M5 can read volumes down to 2uL with the uMax Low. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. Data Acquisition & Analysis Software. com A. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. . The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. 5. 3: The cuvette chamber. . Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. . The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Flexible for 6-well to 384-well microplates. Refer to “Installing Detection Cartridges” in. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. These robust readers have been placed in labs from Antarctica to the. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Step 4. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. D 1 1. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The . The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated.